## Flux Variability Analysis

Use this tool to explore metabolic state of the system. The program computes minimal and maximal flux through the reaction (range of fluxes) consistent with maximal theoretical flux towards selected metabolite. These values are unique and can be used to draw conclusions about internal flux distribution. Calculations will take about 10 minutes. The program will set the flux towards selected metabolite to maximal theoretical value and compute the minimal and maximal flux through each reaction. Results will be displayed, as calculation progresses.

**The output** of the Flux Variability Analysis run contains maximal theoretical growth rate, status of the optimisation (OPTIMAL if the model is feasible) and the table of metabolic flux ranges. Each row contains reaction name, computed minimal flux, computed maximal flux, reaction formula and gene annotation linked to ScoDB, ScoCYC and KEGG.